What is Desktop.ini ?

Have you seen this before? Some of you might have seen this when you configure your Windows setting to show hidden files.

Desktop.ini is a hidden file that used to customize and adjust setting for Windows folder that contain desktop.ini

Can it be deleted?

This file can be safely deleted from any directory.  However, because this file may have settings associated with the folder that it contains and deleting this file will change these settings back to default. For example, if the folder containing this file has a different icon and you delete this file the default folder icon will be re-enabled.

Is this file a virus?

No. By default this file is not a virus. However, there have been a few known viruses that exploit the capabilities of the desktop.ini file. So it can be infected or if created by another program may have attributes that may compromise your computer. If you're concerned about this file being a virus, scan the file with an antivirus program.